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Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection
dc.contributor.author | Ben-Ami, Roni et al. | |
dc.date.accessioned | 2020-07-06T14:38:05Z | |
dc.date.available | 2020-07-06T14:38:05Z | |
dc.date.issued | 2020-05-07 | |
dc.identifier.uri | https://doi.org/10.1101/2020.04.17.20069062 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12663/1960 | |
dc.description.abstract | Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test kits has become a major bottleneck. Here we demonstrate pooling strategies to perform RNA extraction and RT-PCR in pools, significantly increasing throughput while maintaining clinical sensitivity. We implemented the method in a routine clinical diagnosis setting of asymptomatic populations, and already tested 5,464 individuals for SARS-CoV-2 using 731 RNA extraction and RT-PCR kits. We identified six SARS-CoV-2 positive patients corresponding to 0.11% of the tested population. | en_US |
dc.language | English | en_US |
dc.subject | Betacoronavirus | en_US |
dc.subject | Coronavirus | en_US |
dc.subject | COVID-19 | en_US |
dc.subject | Pandemics | en_US |
dc.subject | Real-Time Polymerase Chain Reaction | en_US |
dc.subject | RNA | en_US |
dc.title | Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection | en_US |
eihealth.country | Global (WHO/OMS) | en_US |
eihealth.category | Virus: natural history, transmission and diagnostics | en_US |
eihealth.type | Published Article | en_US |
eihealth.maincategory | Slow Spread / Reducir la Dispersión | en_US |
dc.relation.ispartofjournal | medRxiv | en_US |
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