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dc.contributor.authorBoorman, J. P. T.
dc.contributor.authorPorterfield, J. S.
dc.date.accessioned2023-01-16T13:42:38Z
dc.date.available2023-01-16T13:42:38Z
dc.date.issued1956
dc.identifier.urihttps://academic.oup.com/trstmh/article-abstract/50/3/238/1899150?redirectedFrom=fulltexten_US
dc.identifier.urihttps://hdl.handle.net/20.500.12663/3390
dc.description.abstractA technique employing a mouse skin membrane and heparin-treated blood for infecting mosquitoes and for the demonstration of virus transmission is described. (2) Using this technique, Aedes aegypti mosquitoes were infected with Zika virus. (3) Little or no virus was detectable in the mosquitoes on days 5 to 10, but thereafter the virus level rose and remained steady at approximately 105.0 mouse LD50 per mosquito, from day 20 until day 60. (4) Back-feeding experiments through a mouse skin membrane into uninfected mouse blood resulted in transmission of virus in 12 out of 20 cases. 5. Successful infection of a rhesus monkey by the bites of three mosquitoes was demonstrated 72 days after an infected blood meal. (6) The significance of these findings in the epidemiology of Zika virus infections is discussed.en_US
dc.languageEnglishen_US
dc.subjectZika Research Projecten_US
dc.subjectZika Virusen_US
dc.titleA simple technique for infection of mosquitoes with viruses; transmission of Zika virusen_US
eihealth.countryOthersen_US
eihealth.categoryEpidemiology and epidemiological studiesen_US
eihealth.typeResearch protocol informationen_US
eihealth.maincategorySave Lives / Salvar Vidasen_US
dc.relation.ispartofjournalTransactions of the Royal Society of Tropical Medicine and Hygieneen_US


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