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dc.contributor.authorPardee, Keith
dc.contributor.authorGreen, Alexander A.
dc.contributor.authorTakahashi, Melissa K.
dc.contributor.authoret al.
dc.description.abstractThe recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises. PAPERCLIP.en_US
dc.subjectZika Research Projecten_US
dc.subjectZika Virusen_US
dc.subjectZika Virus Infectionen_US
dc.subjectRNA Virus Infectionsen_US
dc.titleRapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Componentsen_US
eihealth.categoryEpidemiology and epidemiological studiesen_US
eihealth.typeResearch protocol informationen_US
eihealth.maincategorySave Lives / Salvar Vidasen_US
dc.contributor.corporatenameUniversity of Toronto. Leslie Dan Faculty of Pharmacyen_US
dc.contributor.corporatenameThe Biodesign Institute and the School of Molecular Sciences .Biodesign Center for Molecular Design and Biomimeticsen_US
dc.contributor.corporatenameMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.corporatenameBoston University. Department of Biomedical Engineeringen_US
dc.contributor.corporatenameHarvard University. Wyss Institute for Biologically Inspired Engineeringen_US

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